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52Research HighlightsThe fluorescence microscope is a basic tool of biotechnology re-search, and the development of a new form of microscopy is an important key step to advance research in this field. For example, the development of super resolution microscopy* (awarded the 2014 Nobel Prize in Chemistry) has dra-matically improved the resolution of fluo-rescence microscopes (Fig.1). However, this has in turn given rise to problems caused by chromatic aberration (misreg-istration of different light wavelengths) which had hitherto been negligible. Researchers at the NICT Advanced ICT Research Institute have developed a method that can measure and cor-rect chromatic aberration caused not only by the lenses and other compo-nents of a microscope, but also by bio-logical samples being viewed under the microscope. Using this method, the chromatic aberration of super resolu-tion microscopy can be improved ap-proximately tenfold compared with conventional apparatus.With the development of image ac-quisition and computation methods, it “With this tech-nique we can ac hieve a chro-matic aberration correction ac-curacy of about 15 nm in three dimensions.”was possible to acquire images with color shifts caused by chromatic aber-ration in the sample being observed by means of multiple methods such as multi-color staining of the same ob-jects in similar samples, or observation of samples in the fluorescent spectra that are normally cut o (Fig.2).According to Senior Researcher At-sushi Matsuda of the Frontier Research Laboratory, “with this technique we can achieve a chromatic aberration correction accuracy of about 15 nm in three dimensions, allowing accurate interpretation of images obtained by super-resolution microscopy.” As a re-sult, it is now possible to use fluores-cence microscopes to perform mea-surements that could previously only be made using an electron micro-scope. The high-precision chromatic aber-ration correction software developed in this study can be downloaded free of charge.https://github.com/macronucleus/Chromagnon/blob/master/README.mdFig.1 : Example of multicolor observations with a fluorescence microscope5 µmConventional microscopySuper-resolution microscopyYXHigh-precision chromatic aber-ration correction method for su-per-resolution microscopyThe software developed in this study can be downloadedDEVELOP